other:Article Title: Addressing the specific roles of histone modifications in transcriptional repression.
Article Snippet: Membranes were blocked in 5% skimmed milk and probed o/n at 4 °C with primary antibodies against H3K4me0 (Active Motif, cat. no 91317, 1:10 000), H3K4me1 (Thermo Fisher, cat. no 710795, 1:3000), H3K4me2 (Thermo Fisher, cat. no MA5-33383, 1:2000), H3K4me3 (Cell Signaling Technology, cat. no 9751, 1:1000), H3K36me3 (Active Motif, cat. no 61021, 1:2000), WDR5 (Bethyl Laboratories, cat. no A302-429A, 1:1000), histone H4 (Cell Signaling Technology, cat. no 2935, 1:1000).
Article Title: Selective GRP94 inhibitors and uses thereof
Article Snippet: The protein lysates (5-50 pg) were electrophoretically resolved by SDS-PAGE, transferred onto nitrocellulose membranes and probed with the indicated primary antibodies against: HER2 (Zymed, 28004), Hsp70 (Stressgen, SPA-810), Grp94 (Stressgen, SPA-850), Hsp90α (Abcam, Ab2928), Hsp90β (StressMarq, SMC-107B), Grp78 (Cell Signaling, 3183), Raf-1 (Santa Cruz, sc-133), phospho-Raf-1 (Cell Signaling, 9421), MEK1/2 (Cell Signaling, 8727), phospho-MEK1/2 (Cell Signaling, 9154), ERK1/2 (Cell Signaling, 4695), phospho-ERK1/2 (Cell Signaling, 4370), AKT (Cell Signaling, 9272), GM130 (Cell Signaling, 2296), Flotillin 2 (Cell Signaling, 3436), Histone H4 (Cell Signaling, 2592), Histone H1 (Santa Cruz, sc-8030), Caspase 3 (Cell Signaling, 9665), cleaved PARP (Promega, G7341), a-tubulin (Sigma, T5168) and 3-actin (Sigma, A1978).
Article Title: The mammalian Ku70 C-terminus SAP domain is required to repair DNA damage
Article Snippet: The following primary antibodies were used: Ku70 (Invitrogen, MA5-42413; Santa Cruz, sc-56129), Ku80 (Santa Cruz, sc-5280), GAPDH (Santa Cruz, sc-365062), γH2AX (Santa Cruz, sc-517348; Cell Signaling Technology, #2577L), Myc (Santa Cruz, sc-40); XRCC4 (Santa Cruz, sc-365118), XLF (Santa Cruz, sc-393844), LIG4 (Santa Cruz, sc-28232; Cell Signaling Technology, #14649S), total DNA-PKcs (Cell Signaling Technology, #12311S), phosphorylated DNA-PKcs (S2056) (Abcam, ab-18192-100), histone H4 (Cell Signaling Technology, #2935T), and histone H3 (Cell Signaling Technology, #9715).
Article Title: Tumor-derived PRMT1 suppresses macrophage antitumor activity by inhibiting cGAS/STING signaling in gastric cancer cells
Article Snippet: Primary antibodies, including: PRMT1 (CST, Cat#2449), HSP70 (CST, Cat#4872), phospho-TBK1 (CST, Cat#5483), phospho-IRF3 (CST, Cat#4947), cleaved caspase3 (CST, Cat#9661), CD206 (CST, Cat#24595), CD86 (CST, Cat#91882), phospho-Stat1 (CST, Cat#9167), phospho-Stat2 (CST, Cat#88410), H4R3me2a (Invitrogen, Cat#PA5-102612), Histone H4(CST, Cat#2592) and actin (CST, Cat#4970).
Article Title: Tumor-derived PRMT1 suppresses macrophage antitumor activity by inhibiting cGAS/STING signaling in gastric cancer cells.
Article Snippet: Primary antibodies, including: PRMT1 (CST, Cat#2449), HSP70 (CST, Cat#4872), phospho-TBK1 (CST, Cat#5483), phospho-IRF3 (CST, Cat#4947), cleaved caspase3 (CST, Cat#9661), CD206 (CST, Cat#24595), CD86 (CST, Cat#91882), phospho-Stat1 (CST, Cat#9167), phospho-Stat2 (CST, Cat#88410), H4R3me2a (Invitrogen, Cat#PA5-102612), Histone H4(CST, Cat#2592) and actin (CST, Cat#4970).
Article Title: Addressing the specific roles of histone modifications in transcriptional repression
Article Snippet: Membranes were blocked in 5% skimmed milk and probed o/n at 4 °C with primary antibodies against H3K4me0 (Active Motif, cat. no 91317, 1:10 000), H3K4me1 (Thermo Fisher, cat. no 710795, 1:3000), H3K4me2 (Thermo Fisher, cat. no MA5-33383, 1:2000), H3K4me3 (Cell Signaling Technology, cat. no 9751, 1:1000), H3K36me3 (Active Motif, cat. no 61021, 1:2000), WDR5 (Bethyl Laboratories, cat. no A302-429A, 1:1000), histone H4 (Cell Signaling Technology, cat. no 2935, 1:1000).
Ubiquitin Proteomics:Article Title: Overexpression of ATase1 and ATase2 disrupts the secretome and causes a progeria phenotype
Article Snippet: .. The following primary antibodies were used in this study: β-actin (#3700, 1:5,000; Cell Signaling; #4967, 1:5,000; Cell Signaling), ATase1/NAT8B (#PA5-77166, 1:1,000; Invitrogen), ATase2/NAT8 (#NBP1-47863, 1:1,000; NOVUS), Acetyl-Histone H2A Lys5 (#2576, 1:1,000; Cell Signaling), Acetyl-Histone H2B Lys5 (#12799, 1:1,000; Cell Signaling), Acetyl-Histone H3 Lys9 (#9649, 1:1,000; Cell Signaling), Lys14 (#7627, 1:1,000; Cell Signaling), Lys18 (#13998, 1:1,000; Cell Signaling), Lys27 (#8173, 1:1,000; Cell Signaling), Lys56 (#4243, 1:1,000; Cell Signaling), Acetyl-Histone H4 Lys5 (#8647, 1:1,000; Cell Signaling), Lys8 (#2594, 1:1,000; Cell Signaling), Lys12 (#13944, 1:1,000; Cell Signaling), Histone H2A (#12349, 1:1,000; Cell Signaling), Histone H2B (#12364, 1:1,000; Cell Signaling), Histone H3 (#4499, 1:1,000; Cell Signaling), Histone H4 (#13919, 1:1,000; Cell Signaling), and Ubiquitin (#3936, 1:1,000; Cell Signaling). .. Both donkey anti-rabbit and goat anti mouse IRDye 800CW and 680RD-conjugated secondary antibodies (#926-32213; #926-68070; #926-32210; #926-68073; LI-COR) were used for infrared imaging (LI-COR Odyssey Infrared Imaging System; LI-COR Biosciences).
Article Title: Overexpression of ATase1 and ATase2 disrupts the secretome and causes a progeria phenotype.
Article Snippet: .. The following primary antibodies were used in this study: β-actin (#3700, 1:5,000; Cell Signaling; #4967, 1:5,000; Cell Signaling), ATase1/NAT8B (#PA5-77166, 1:1,000; Invitrogen), ATase2/ NAT8 (#NBP1-47863, 1:1,000; NOVUS), Acetyl-Histone H2A Lys5 (#2576, 1:1,000; Cell Signaling), Acetyl-Histone H2B Lys5 (#12799, 1: 1,000; Cell Signaling), Acetyl-Histone H3 Lys9 (#9649, 1:1,000; Cell Signaling), Lys14 (#7627, 1:1,000; Cell Signaling), Lys18 (#13998, 1: 1,000; Cell Signaling), Lys27 (#8173, 1:1,000; Cell Signaling), Lys56 (#4243, 1:1,000; Cell Signaling), Acetyl-Histone H4 Lys5 (#8647, 1: 1,000; Cell Signaling), Lys8 (#2594, 1:1,000; Cell Signaling), Lys12 (#13944, 1:1,000; Cell Signaling), Histone H2A (#12349, 1:1,000; Cell Signaling), Histone H2B (#12364, 1:1,000; Cell Signaling), Histone H3 (#4499, 1:1,000; Cell Signaling), Histone H4 (#13919, 1:1,000; Cell Signaling), and Ubiquitin (#3936, 1:1,000; Cell Signaling). .. Both donkey anti-rabbit and goat anti mouse IRDye 800CW and 680RDconjugated secondary antibodies (#926-32213; #926-68070; #926- 32210; #926-68073; LI-COR) were used for infrared imaging (LICOR Odyssey Infrared Imaging System; LI-COR Biosciences).
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